It is not recommended to use plastic buckets on UV fish spectrometers. Technically, any standard 10×10mm bucket can be used, but you need to know the material and transmission profile before doing so. Around 300 nm, even UV plastic buckets begin to absorb light and affect the results.
Excellence buckets provide exceptional window parallelism and offer small optical path length tolerances of just ± 0.01 millimeters. This enables them to deliver the precision needed for pharmaceutical and research applications. Made of JGS1 UV grade molten spectrophotometer cuvettes silica, the onset of absorption occurs below 170 nm and there are no absorption bands between 170 nm and 250 nm. Exceptionally high transmission over UVB, UVA, visible spectrum and near IR. The transmission spectra of our UV quartz cells can be seen below.
Run the solution on the glass wall towards the bucket to prevent bubble formation. In a standard spectrometer box, the path of the light or the length of the path is the internal distance from the front window to the rear window. The length of the optical path of the standard bucket is 10 mm. Both standard and excellence buckets are designed for long-term use.
The distance traveled by the light through the sample is known as path length. The length between the inner walls of a UV fish bucket where light passes is known as the path of light. The internal distance between the front and rear windows of a conventional spectrophotometer box is the path of the light or the length of the path. Usually a UV fish bucket is chosen with exact dimensions, such as a path length of 10 mm.
However, there is a chance that the samples are too diluted and it is not possible to obtain sufficient absorption with standard-sized buckets. Of course, the solution can concentrate the samples, but it is usually difficult in situations where the sample evaporates or undergoes a chemical change during the concentration process. The light path or path length of a typical spectrophotometer box is the internal distance from the front window to the rear window of the transparent walls of the bucket. With a wall thickness of 1.25 mm, the standard outer dimensions of a bucket are 12.5 x 12.5 m, height 45 mm.
If you want to choose one, you need to define the specific use and equipment. For example, plastic may not be efficient for UV range experiments, but it is a cost-effective alternative to all visible light studies. UV quartz veins are designed to contain samples for spectroscopic experiments. We offer both quartz semimers with two polished sides for absorption spectroscopy and quarter-semimers with four polished sides for fluorescence spectroscopy. For measurement best practices, the bin should remain on the bin holder between measurements. If it is removed, it should be ensured that the bucket is always placed in the same direction on the bucket holder, i.e. with the label to the light source.
You get the affordable full range of UV quartz material, the $12.3 price tag for QS2002 makes it a great buy for labs on a budget. For UV-VIS absorption studies you absolutely need a UV quartz tammmer, if you cut corners here when getting a cheap glass or plastic cell, your data will be inaccurate, it is not advisable to do this. This baking material is suitable for the visible spectrum and has a decent transmission range of 340-2,500 nm. In this article, we will introduce the basic material requirements, and then we will draw a contrast between the different types of buckets to help you select the right bucket.